Western
Meeting of Poultry
Clinicians and Pathologists
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Clinicians and Pathologists
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Upregulation of a Novel Chicken TLR Following Salmonella enterica Serovar Typhimurium Infection Rowan Higgs1,2, Paul Cormican1, Sarah Cahalane1, Brenda Allan3, Andrew T. Lloyd1, Kieran Meade1, Tharappel James1,4, Lorne Babiuk3 and Cliona O’Farrelly1,5 Toll-like Receptors (TLRs) are a group of highly conserved molecules that initiate the innate immune response to pathogens by recognizing structural motifs expressed by microbes. We have identified two novel chicken TLRs by a bioinformatic search of the chicken genome. TLR13 and TLR14 are found on chromosomes 11 and 3 respectively. TLR13 mRNA was expressed in the spleen, tongue, bursa, caecum and bone marrow of healthy chickens. This broad expression suggests a role for this novel receptor in constitutive host defense. TLR14 mRNA was expressed in the spleen, bursa and bone marrow of healthy chickens. Following Salmonella enterica serovar Typhimurim infection, no change in expression of TLR13 was observed, but real time PCR demonstrated significant upregulation of TLR14. Interestingly, induction of TLR14 expression following infection was highly similar with that of TLR2, suggesting a functional relationship between these two receptors.
1. Education and Research Centre, St. Vincent’s University Hospital, Dublin 4, Ireland. 2. Department of Medicine, University College Dublin, Belfield, Dublin 4, Ireland. 3. Vaccine and Infectious Disease Organization, Saskatoon, Saskatchewan, S7N 5E3 4. Moyne Institute of Preventive Medicine, Trinity College Dublin, Dublin 2, Ireland. 5. Conway Institute, University College Dublin, Belfield, Dublin 4, Ireland.
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